At present, data comparing the quantification methods for measurement of free vitamin D (direct assay [direct 25-OHDF] and estimated by calculation [calculated 25-OHDF]), are scarce. The aim of this study was to analyse the concordance between these two methods of 25-OHDF analysis (direct vs. calculated). Methods Serum values of total 25-OHD (25-OHDT), vitamin D binding protein (DBP) (by R&D Systems ELISA), calculated 25-OHDF (by DBP, albumin and 25-OHDT) and direct 25-OHDF (by DIAsource ELISA) were analysed in 173 healthy women (aged 35–45 years). Assessment of concordance was evaluated by the Bland-Altman plot and the total deviation index (TDI). Results The mean values of calculated and direct 25-OHDF in these subjects were 5.27 ± 2.5 and 3.83 ± 1.01 pg/mL, respectively. We found significantly lower values of 25-OHDF on comparing subjects with and without vitamin D deficiency, independently of the method used. The total deviation index evaluated by the Bland-Altman plot showed low concordance for both measurements. Only low 25-OHDF levels were concordant. Conclusions This study shows that the concordance between these two methods of 25-OHDF analysis is low and has a concentration dependent bias. Further studies are necessary to clarify the reference values and the indications for 25-OHDF measurement.
CITATION STYLE
Peris, P., Filella, X., Monegal, A., Guañabens, N., Foj, L., Bonet, M., … Carrasco, J. L. (2017). Concordance between direct and indirect measurements of free 25-OH vitamin D. Clinica Chimica Acta, 475, 169–171. https://doi.org/10.1016/j.cca.2017.10.016
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