Use of green fluorescent protein to monitor lactobacillus plantarum in the gastrointestinal tract of goats

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Abstract

The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administra­tion. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.

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Han, X., Wang, L., Li, W., Li, B., Yang, Y., Yan, H., … Chen, Y. (2015). Use of green fluorescent protein to monitor lactobacillus plantarum in the gastrointestinal tract of goats. Brazilian Journal of Microbiology, 46(3), 849–854. https://doi.org/10.1590/S1517-838246320140556

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