Cloning and Expression of Lactate Dehydrogenase H Chain Gene in Adipose Tissues of Korean Cattle

Abstract

To understand molecular mechanisms that regulate deposition and release of intramuscular fat, a fasting-induced c was identified by differential screening from cDNA library of adipose tissues of Korean cattle. The clone had a total length of 1, nucleotides coding for 334 amino acids, It was identified as one encoding L-lactate dehydrogenase H chain (LDH-B). Comparison of deduced amino acid sequences of bovine LDH-B with those of pig, human, rat, and mouse showed 98%, 98%, 97%, and 96% iden respectively. Food deprivation for 48 h increased mRNA levels of LDH-B gene in adipose tissues of Korean cattle compared to fed-6 h refed- tissues. The expression of obese mRNA was examined for individual adipose tissue from several fat depots. Fasting indu expression of LDH-B gene in subcutaneous adipose tissues, but it did not affect expression levels in abdominal, perirenal intramuscular tissues. Results demonstrate that induction of LDH-B gene during fasting may represent a metabolic shift from anaero state to aerobic predominance in fasted adipose tissues and that its responses to fasting are different among several adipose tiss (Asian-Aust. J. Anim. Sci. 2001. Vol 14, No. 12 : 1670-1674).