Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.
CITATION STYLE
Celli, S., Breart, B., & Bousso, P. (2008). Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes. Methods in Molecular Biology (Clifton, N.J.), 415, 119–126. https://doi.org/10.1007/978-1-59745-570-1_7
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