Construction of promoter-probe shuttle vectors for Escherichia coli and corynebacteria on the basis of promoterless α-amylase gene

Abstract

We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless α-amylase gene originating from Bacillus subtilis Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. α-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of α-amylase activity.