Production of lily symptomless virus-free plants by shoot meristem tip culture and in vitro thermotherapy

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Abstract

To produce virus-free plants, a simple and original protocol was established by combining several techniques: repeated shoot meristem excision before and during in vitro culture and thermotherapy applied to bulblets in vitro. Lily symptomless virus (LSV) is a major virus that decreases plant growth vigor and the quality of cut flowers, yet infected plants show no distinct symptoms. Stock bulbs of pollenless Asiatic hybrid lily (L. x elegans Thunb) lines ('409' and '599') were used as explant. Shoot meristems were excised and micropropagated. Thermotherapy (42 days at 35°C) was applied to in vitro growing bulblets and a second meristem cut was then made from heat-treated material. Leaf tissues from bulblets formed before or postheat treatments were analyzed either by enzyme-linked immunosorbent assay or by reverse transcriptase-polymerase chain reaction. Line '499' produced LSV-free plants without heat treatment, but line '599' produced LSV-free plants only after heat treatment. The virus-free lily bulblets grew vigorously in vitro and acclimatized promptly. It is suggested that thermotherapy given to in vitro growing bulblets effectively eliminated the virus and induced a fast and efficient micropropagation technique for virus-free mother plant stock.

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Nesi, B., Trinchello, D., Lazzereschi, S., Grassotti, A., & Ruffoni, B. (2009). Production of lily symptomless virus-free plants by shoot meristem tip culture and in vitro thermotherapy. HortScience, 44(1), 217–219. https://doi.org/10.21273/hortsci.44.1.217

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