Diaphragma frozen homograft for rats' abdominal wall defects repair

Abstract

Objective: To analyse the morphology and biomechanics of diaphragma frozen homografts used for rats' abdominal wall defects repair. Method: Thirty wistar rats with abdominal wall resection and reconstruction using frozen diaphragma homografts were compared to twenty other rats submitted to abdominal wall incision and closure (control). Animals were euthanized after 3 and 6 months and abdominal walls were avaliated histologically with H/E and Picrosiriud Red staining and tensiometric evaluation. Results: The tensiometric parameters were greater in the experimental group at 3 months after surgery. The percentage of mature collagen was significantly greater at 6 months after surgery in control and experimental groups. Tensiometric parameters and type I collagen as well maturation collagen index and rupture maximal strength were statistically correlated after 3 and 6 months. Conclusion: Frozen diaphragma homograft can be an alternative to repair the defects of abdominal wall.