Abstract
Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations. © 2014 Nature America, Inc. All rights reserved.
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CITATION STYLE
Shen, B., Zhang, W., Zhang, J., Zhou, J., Wang, J., Chen, L., … Skarnes, W. C. (2014). Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nature Methods, 11(4), 399–402. https://doi.org/10.1038/nmeth.2857
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