Tm7sf2 may participate in the healing of burn wounds

Abstract

The transmembrane 7 superfamily member 2 (Tm7sf2) gene been reported to be involved in the reduced cholesterol levels of patients with large burn areas. To investigate the importance of Tm7sf2 in the burn wound healing process, a total of 10 Sprague-Dawley rats underwent electrical burns. Blood serum was collected for the culture of HaCaT human keratinocyte cells. Tm7sf2 small interfering RNAs (siRNAs) were prepared and transfected into the normal- and burn serum-cultured HaCaT cells. Monocyte-endothelial cellular adhesion ability and cell proliferation, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the levels of autophagy proteins Beclin1 and LC3-II, which were detected by western blot assay and real time-quantitative polymerase chain reaction, and it was identified that Tm7sf2 siRNAs were successfully transfected. Cell proliferation was reduced and monocyte-endothelial cell adhesion increased in the burn serum group compared with the normal serum group (P