Certification of a New Naringenin Reference Material by Coulometric Titrimetry Combined HPLC-MS

Abstract

Certified reference material (CRM) is a kind of measuring tool with high accuracy and traceability. CRMs are the important guarantee of accurate and reliable results of analysis methods. They are the medium of international communication of experimental data. CRMs are widely used in various fields and play a pivotal role in analytical science. However, in the characterization of CRMs, available absolute analysis methods are very limited. In this situation, accurate and reliable relative analysis methods should be applied. In this work, coulometric titrimetry (CT) was applied to the characterization of flavone naringenin CRM according to its structure. For further illustration of the principle of CT, high-performance liquid chromatography tandem mass spectrometry (HPLC-MS) was utilized to analyze the titration products in detail. As the conclusion of this paper, the advantages and the influence factors of CT were given. Mass balance (MB) method was employed to verify the result of CT, and consistent result was obtained.