Isolation and Identification of Streptococcus pyogenes from Patients and Objects in Hospital Environment in Thi-Qarcity in Iraq

Abstract

This study aimed to screen Streptococcus pyogenes in the hospital and investigate the incidence of thisbacteria. The genotypes of the S.pyogenes in the study samples will be determined using 16Sr RNA and somevirulence factors(emm, scpA, speA genes).The majority of the samples (74.1%) were isolated from patientsat the emergency room and (25.9%) were from hospital environment (objects).The current study showed thatthe total of 215 patients with pharyngitis were included 118 (55%) males higher than 97 (45%) females. Thepatients’ ages ranged from 2 to 62 years, which were divided into four categories. The majority of patients65 (30.1%) of patients from 3 to 6 years old, 54 (25%) of patients less than 3years, 51 (23.6%) from 7 to 11years old, and 46 (21.3%) more than 12years. Six isolates sent for sequencing were after that submission inNCBI-GenBank database. The results of nucleotide sequence alignment of the isolates revealed that thereare multiple point mutations, four of them appeared as a single nucleotide polymorphism (SNP) and twoappeared in duplicate. Polymorphic sites (Mutations) of 6 S.pyogenes isolates.