Here we describe a novel multicolor bioluminescent imaging platform that enables us to simultaneously investigate transcription factor nuclear factor-κB (NF-κB) signalling and apoptosis. We genetically modified the human breast cancer cell line MDA-MB-231 to express green, red, and blue light-emitting luciferases to monitor cell number and viability, NF-κB promoter activity, and to enable specific cell sorting and detection, respectively. Z-DEVD-animoluciferin, the pro-luciferin substrate, was used to determine apoptotic caspase 3/7 activity. We used this multicolored cell line for the in vitro evaluation of natural compounds and in vivo optical imaging of tumor necrosis factor (TNFα)-induced NF-κB activation (Mezzanotte et al.,PLoS One 9:e85550, 2014).
CITATION STYLE
Knol-Blankevoort, V. T., Mezzanotte, L., Rabelink, M. J. W. E., Löwik, C. W. G. M., & Kaijzel, E. L. (2016). Development of a multicolor bioluminescence imaging platform to simultaneously investigate transcription factor NF-κb signaling and apoptosis. In Methods in Molecular Biology (Vol. 1461, pp. 255–270). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3813-1_21
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