Development and validation of RP-HPLC assay for levofloxacin in rat plasma and saliva: Application to pharmacokinetic studies

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Abstract

A new, simple, specific, accurate and precise reversed phase-high performance liquid chromatography (RP-HPLC) method was developed for the determination of levofloxacin in rat plasma and saliva was developed. An HPLC system based on a Phenomenex Luna C18 Column (250 × 4.6 mm) and a UV detector (λ = 296 nm) were used. A mixture of Acetonitrile: water (80:20 v/v) adjusted to pH 3.5 by orthophosphoric acid at a flow rate of 1.4 ml/min was used as mobile phase. The proteins were precipitated with methanol. The average recovery was 94.79 and 92.66%, respectively in plasma and saliva. The detection limit for levofloxacin in plasma and saliva was 1 μg/ml. The calibration curve was linear over the concentration range 1 to 16 μg/ml for plasma and saliva. The inter-day and intra-day assay coefficients of variation were found to be less than 5%. The present validated method was successfully used for pharmacokinetic studies of levofloxacin in plasma and saliva. ©2011 Academic Journals.

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Sheikh, N. W., Tripathi, A. S., Chitra, V., Choudhury, A., & Dewani, A. P. (2011). Development and validation of RP-HPLC assay for levofloxacin in rat plasma and saliva: Application to pharmacokinetic studies. African Journal of Pharmacy and Pharmacology, 5(13), 1612–1618. https://doi.org/10.5897/AJPP11.001

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