Circulating tumor cells (CTCs) have been implicated as the seeds of cancer metastasis and therefore have the potential to provide significant prognostic and diagnostic values. Here, we describe a procedure for separating CTCs from whole blood based on size and deformability using the microfluidic ratchet device. This device leverages the ratcheting motion of single cells created as they are deformed through funnel-shaped constrictions using oscillatory flow in order to divert cells based on differences in size and deformability. Subsequent methods for CTC identification and enumeration using immunofluorescence after separation are also described.
CITATION STYLE
Park, E. S., Duffy, S. P., & Ma, H. (2017). Microfluidic separation of circulating tumor cells based on size and deformability. In Methods in Molecular Biology (Vol. 1634, pp. 21–32). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7144-2_2
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