Identifi cation of low-expressing transcripts of the NPY receptors’ family in the murine lingual epithelia

Abstract

Detection of low-expressing transcripts in tissues is a major technical challenge that requires considerate advance planning. To produce high-quality publishable data, many controls need to be employed, including knock out animal models, independent assays, and high-end imaging techniques. The current protocol describes the use of commercial kit, QuantiGene ViewRNA 1-plex assay for a reliable detection of lowexpressing transcripts in formalin-fi xed paraffi n-embedded murine tissues. Examples of positive (highexpressing) and negative (knock out) control samples are provided to describe a case study.