Molecular genetic methods have several advantages over classical morphological and chemical analyses. The genetic method requires genotype instead than phenotype, therefore PCR-based techniques have been widely used for a rapid identification of plant species, varieties and chemotypes. Recently, the molecular discrimination of some higher plant species has been evaluated using sequences of a 5S-rRNA gene spacer region. The variation in the nontranscribed sequence (NTS) region has been used in a number of plant species for studying intraspecific variation, genome evolution, and phylogenetic reconstruction. Here, we describe a rapid method based on the use of the 5S-rRNA-NTS region as a tool for plant DNA fingerprinting, which combines PCR, sequencing and restriction fragment length polymorphism analyses. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Bertea, C. M., & Gnavi, G. (2012). Restriction fragment length polymorphism of the 5S-rRNA-NTS Region: A rapid and precise method for plant identification. Methods in Molecular Biology, 862, 89–101. https://doi.org/10.1007/978-1-61779-609-8_8
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