Bacillus subtilis C2 producing lipase isolated from bulk shrimp paste in Samarinda East Kalimantan

Abstract

Lipase is an enzyme that catalyzes the hydrolysis reaction of lipid (triacylglycerol) to glycerol and free fatty acids which has been used in various industrial applications such as food, cosmetics, detergents and pharmaceuticals. Bacillus subtilis C2 isolated from bulk shrimp paste (terasi) in Samarinda East Kalimantan, it showed producing lipase, and able to degrade fat. Lipase activity was determined titrimetrically. The ability of the strain to degrade fatty substrates was investigated in the medium 0.1% of olive oil, virgin coconut oil (VCO), cooking oil, tween 80, and Schleicheraoleosa oil. The activities of lipase treated based on the period of incubation, temperature, and pH. The results showed that the optimum activity of lipase was 1.09 U/mL after five days incubation, 1.43 μmol/mL at 30°C and 1.96 U/mL at pH 9.0.The enzyme gave the highest lipase activity 1.76 μmol/mL used olive oil as a substrate. The physiology analysis showed the bacterium B. subtilis C2-lipolytic was thermotolerant, halotolerant and strictly aerobic.