Comparative study of Cladophora sp. cellulose by using FTIR and XRD

Abstract

Cellulose Crystalline Cladophora sp. (CCC) was successfully isolated from the freshwater algae Cladophora sp. by used the hydrolysis method. The alkaline treatment can affect the structure of CCC that is showed by the spectrum of Fourier Transform Infrared (FTIR). The typical range for lignin does not appear here, which indicates the delignification process using NaOH plus hydrolysis of HCl has succeeded in removing lignin. The spectrum associated with functional group impurity (1800-1050 cm-1) with lipid, protein, and nucleid acid content are still seen after alkaline treatment and acid hydrolysis. Analysis of crystallinity with X-ray Diffraction (XRD) data showed that CCC had the highest crystallinity index on CD-K (93.4%), Cd-P (66.6%), and Cd-S (63.04%). The smallest particle size for each cellulose is Cd-K (9.5 nm), Cd-S (25.23 nm), and Cd-P (49.57 nm). Based on these results, the CCC production from Cladophora sp. using alkaline and acid hydrolysis treatment is enough to get samples with high crystallinity. The CCC product has the potential to be an excellent reinforcing material for biomaterial-based polymer materials.