We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample. © 2009 Piqueur et al; licensee BioMed Central Ltd.
CITATION STYLE
Piqueur, M. A. C., Verstrepen, W. A., & Bruynseels, P. (2009). Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA. Virology Journal, 6. https://doi.org/10.1186/1743-422X-6-95
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