Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture.
CITATION STYLE
Sydow, H. G., Pieper, T., Viebahn, C., & Tsikolia, N. (2017). An early chick embryo culture device for extended continuous observation. In Methods in Molecular Biology (Vol. 1650, pp. 309–317). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7216-6_21
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