Identification of TNF-α-308G/A (rsl800629) polymorphism in Bangladeshi population related to type-2 diabetes

Abstract

This study was designed to inspect the association between tumor necrosis factor (TNF)-α-308G/A (rsl800629) polymorphism with diabetes mellitus type-2 (DMT2) in Bangladeshi population. Besides, the role of TNF-α-308G/A in early proliferation of DMT2 has been investigated. The allelic frequency of TNF-α-308G/A and their association with DMT2 was studied using high resolution melting (HRM) analysis and confirmed using sequencing. A few demographic risk factors associated with DMT2 were also investigated routinely. The significance of these risk factors was analyzed statistically. We have analyzed 657 individuals who were distributed into two groups: 330 non-diabetic controls and 327 DMT2 individuals. HRM analysis shows that 11 individuals bare G/A and 2 bare A/A genotype in DMT2 patients. Within non-diabetic individual, we found only one with G/A genotype. The frequency of TNF-α-308G>A are within the Hardy–Weinberg equilibrium (0.00482) at 95% confidence level. TNF-α-308G>A frequency in two age group based on first time diagnosed, we found association with early proliferation of DMT2 with a p-value of 0.008965 in Fisher’s test at 95% confidence level. Our result suggested that the single nucleotide polymorphisms TNF-α-308G>A is closely associated with DMT2 patients in Bangladeshi population. Besides, presence of TNF-α-308G>A polymorphism increases the risk of early proliferation of DMT2.