Enhanced anti-inflammatory effect of polyherbal formulation (MKA) comprising of three selected plants in lipopolysaccharide (LPS) -induced raw 264.7 macrophage cell line

Abstract

Antiinflammatory potential of Polyherbal formulation comprising of three selected plants Mimusops elengi L., Kedrostis foetidissima (Jacq.) Cogn. and Artemisia vulgaris L. MKA were studied in Lipopolysaccharide induced RAW 264.7 macrophage cell line. Initially, the MTT cell viability assay was performed to assess the safety level of MKA for anti-inflammatory studies. The MKA was studied for its anti-inflammatory property in selected four different concentrations (25µg/ml, 50 µg/ml, 75 µg/ml and 100 µg/ml). It was observed that increase in MKA concentration decreased the nitrite release from Lipopolysaccharide induced macrophage cells. Microscopic analysis also revealed that MKA restored the normal cell morphology by reducing the cell damage caused by Lipopolysaccharide. The intracellular reactive oxygen species was detected by 2'-7'-Dichlorodihydrofluorescein diacetate labelling and flow cytometry. It was found that, MKA decreased the intracellular Reactive oxygen species level in Lipopolysaccharide stimulated macrophages in a dose-dependent manner, which proved its enhanced anti-inflammatory effect.