Time-Resolved Evanescent Wave-Induced Fluorescence Anisotropy Measurements

Abstract

The technique of time-resolved evanescent wave-induced fluorescence spectroscopy (TR-EWIFS) has been extended to incorporate fluorescence anisotropy measurements. We report on the application of these time-resolved evanescent wave-induced fluorescence anisotropy measurements (EW-TRAMs) as a source of information concerning the motion and conformational changes of macromolecules that can occur near a solid/liquid interface. We have applied EW-TRAMs to studies of the adsorption of proteins and polymers onto silica from solution. We also discuss the implications and potential complications of the polarisation properties of the standing evanescent field and how these properties can be used to advantage