Abstract
In any form of microscopy, one needs not only an imaging system with enough resolution to delineate the fine details of the specimen but also a suitable contrast mechanism by which to see the shape of the structures of interest. Contrast is the difference between the signal in one pixel and that in another that conveys to the viewer information about the shape of the specimen. It is the difference between a blank screen and an image. © 2006, 1995, 1989 Springer Science+Business Media, LLC. All rights reserved.
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CITATION STYLE
Cheng, P. C. (2006). The contrast formation in optical microscopy. In Handbook of Biological Confocal Microscopy: Third Edition (pp. 162–206). Springer US. https://doi.org/10.1007/978-0-387-45524-2_8
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