High glucose: Activating autophagy and affecting the biological behavior of human lens epithelial cells

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Abstract

• AIM: To clarify the effect of autophagy on human lens epithelial cells (HLECs) under high glucose conditions. • METHODS: HLECs were cultured with different concentrations of glucose and 3-methyladenine (3-MA); the expression of autophagy-related protein LC3B was detected by Western blotting and immunofluorescence histochemistry. The migration of HLECs was quantified by scratch wound assay and the expression of transforming growth factor-β (TGF-β) was measured by real-time polymerase chain reaction. • RESULTS: Compared with 5 mmol/L normal glucose treatment, 40 mmol/L glucose treatment can significantly increase the generation of autophagosome in HLECs, which could be inhibited by 0.375 mmol/L 3-MA treatment. The migration of HLECs and the expression of TGF-β in HLECs induced by high glucose were significantly suppressed by 0.375 mmol/L 3-MA treatment. • CONCLUSION: Autophagy promotes HLECs cell migration and increases the expression of TGF-β after exposed to high glucose, which may relate to the development of diabetic cataract.

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Li, D., Liu, G. Q., & Lu, P. R. (2019). High glucose: Activating autophagy and affecting the biological behavior of human lens epithelial cells. International Journal of Ophthalmology, 12(7), 1061–1066. https://doi.org/10.18240/ijo.2019.07.02

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