The production of cinnamic acid secondary metabolites through in vitro culture of callus Camellia sinensis L with the elicitor of cobalt metal ions

Abstract

Cinnamic acid is one of the secondary metabolites found in Camellia sinensis L plants which can be bioactive. These Cinnamic acid bioactives are useful as anti-cancer, antioxidants, antibacterial, corrosive and as an inducer to increase secondary metabolites in biotechnology's field. The problem of cinnamic acid production from plants constrained by climate change, weather and narrow land conditions with uncontrolled population explosions. The purpose of this study was to obtain cinnamic acid's method production with in vitro culture techniques. The methods used to achieve these objectives: 1) planting shoots of Camellia sinensis L leaf explants, 2) increasing cinnamic acid in callus culture, 3) Qualitative observation of callus growth. 4) Quantitative observation of cinnamic acid content in callus. The results of the study were shaped with increased wet weight callus containing cinnamic acid. The significant finding in this study is that with the addition of cobalt metal ion elicitors, the secondary metabolite of cinnamic acid was obtained by 11.9%. This study implies that the use of the in vitro culture technique of Camellia sinensis L in a relatively short time can produce secondary metabolites of cinnamic acid which increase with the cobalt metal ions elicitor.