Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing

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Abstract

We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units. © 2013, American Society for Microbiology.

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VanInsberghe, D., Hartmann, M., Stewart, G. R., & Mohn, W. W. (2013). Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing. Applied and Environmental Microbiology, 79(6), 2096–2098. https://doi.org/10.1128/AEM.03112-12

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