Phytoconstituents Investigation on the Ethanolic Extract of Azadirachta indica var. Indonesian and Philippines

Abstract

In the present study, phytochemical screening of ethanolic extracts of Azadirachta indica leaves from Indonesia and the Philippines revealed the presence of different phytoconstituents. Preliminary qualitative chemical test, TLC and LC-MS were used. TLC for all the extracts showed bands in long UV 366 for presence of flavonoid, tannin, saponin, terpenoid. As a result of LC-MS analysis of ethanolic extract Azadirachta indica leaves from Indonesia and Philippines, 10 compounds from Indonesian varian and 7 compounds from the Philippines varian were detected using m/z value. In conclusion, phytochemical screening based on TLC and LC-MS/MS show diverse bioactive compounds in ethanolic extract Azadirachta indica leaves from Indonesia and the Philippines. These can be effective approach for selecting best quality of varian leaves and planting area.