Mouse models of immunology are frequently used to study host responses to poxviruses or poxvirus-based recombinant vaccines. In this context, the magnitude of CD8 + T cell responses is often of interest. Methods to evaluate CD8 + T cell responses extend from those that rely on indirect measurement of effector function only, such as cytotoxicity assays, to those that only measure antiviral CD8 + T cell numbers and not function, like peptide MHC tetramers. In this chapter, five methods are provided that cover this range: DimerX staining (a variant of peptide-MHC tetramers), intracellular cytokine staining for interferon-γ, CD62L/Granzyme B staining, and in vitro and ex vivo cytotoxicity assays. We also include tables of vaccinia virus peptide epitopes for use in most of these assays. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Flesch, I. E. A., Wong, Y. C., & Tscharke, D. C. (2012). Analyzing CD8 T cells in mouse models of poxvirus infection. Methods in Molecular Biology, 890, 199–218. https://doi.org/10.1007/978-1-61779-876-4_12
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