Preparation of gold nanocluster bioconjugates for electron microscopy

Abstract

In this chapter, we describe types of gold nanoparticle-biomolecule conjugates and their use in electron microscopy. Included are two detailed protocols for labeling an IgG antibody with gold monolayer protected clusters. The first approach is a direct bonding approach that utilizes the ligand place exchange reaction. The second approach describes NHS-EDC coupling of Au 144(pMBA)60 with IgG. Also included are various characterization techniques for determining labeling efficiency. © 2013 Springer Science+Business Media, LLC.