The Plant Endoplasmic Reticulum: Methods and Protocols

Abstract

Metabolons are protein complexes that contain all the enzymes necessary for a metabolic pathway but also scaffolding proteins. Such a structure allows efficient channeling of intermediate metabolites from one active site to the next and is highly advantageous for labile or toxic intermediates. Here we describe two methods currently used to identify metabolons via protein–protein interaction methodology: immunopre- cipitations using GFP-Trap®_A beads to find novel interaction partners and potential metabolon compo- nents and FRET-FLIM to test for and quantify protein–protein interactions in planta. Key