Desenvolvimento de metodologia verde por HPLC/UV para determinação de glifosato em amostras ambientais de solo

Abstract

Glyphosate, N-(phosphonomethyl)glycine, is a systemic herbicide and post-emergent with great efficiency in the removal of weeds, featuring low cost, which is reflected on its wide application. Due to this fact, special care in order to not contaminate the environment has to be attended, since there is evidence of deleterious effects on the environment. Therefore, the aim of this study was to develop an analytical green methodology for the determination of glyphosate in soil samples using the technique of high performance liquid chromatography. The proposed method describes an efficient alternative for the determination of glyphosate in soil samples by eliminating the step of clean-up. The limits of detection and quantification related to the method were 9.93 and 30.1 Μg L-1. Samples of soil results in recovery of glyphosate ranged from 78.1 to 113.2% due to the percentage fraction of organic, inorganic, among other factors.