Protocol for regeneration in vitro of Arachis hypogaea L.

Abstract

The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage.