Abstract
The 6 x His affinity tags have proved invaluable for the exclusive purification of proteins, in Escherichia coli, of genes cloned in frame with a 6 x His tag and a strong inducible promoter. Here, we demonstrate that the system can be extended to Gram-negative bacteria other than E. coli, by the use of compatible broad-host-range plasmids. As an example, the inducible synthesis and specific purification of the Pseudomonas 6 x His-PfrA siderophore regulatory protein in Pseudomonas putida WCS358 is demonstrated.
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CITATION STYLE
Bertani, I., Devescovi, G., & Venturi, V. (1999). Controlled specific expression and purification of 6ÃHis-tagged proteins in Pseudomonas. FEMS Microbiology Letters, 179(1), 101–106. https://doi.org/10.1111/j.1574-6968.1999.tb08714.x
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