A yeast two-hybrid screening system was developed to screen for small molecules that inhibit the interaction of the Ras and the Raf proteins. Hyperpermeable yeast strains useful for high-throughput screening (HTS) for the two-hybrid system were created. Differential inhibition of the Ras-Raf vs the hsRPB4-hsRPB7 interaction allowed the identification of selective inhibitors.
CITATION STYLE
Khazak, V., Golemis, E. A., & Weber, L. (2005). Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors. Methods in Molecular Biology (Clifton, N.J.), 310, 253–271. https://doi.org/10.1007/978-1-59259-948-6_18
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