Stable knockdown of estrogen receptor a by vector-based RNA interference suppresses proliferation and enhances apoptosis in breast cancer cells

Abstract

Breast cancer, the most common malignancy in women, has a known association with the steroid hormone estrogen. Estrogen receptor a (ERα) plays an important role in the clinical care of breast cancer patients, both as a prognostic factor and as a therapeutic target. Here, we show that a small interfering RNA (siRNA) against ERα downregulates ERa expression in human MCF-7 and Bcap-37 breast cancer cells, causing a significant decrease in breast cancer cell proliferation. Tumor cells lacking ERα expression grew at a much slower rate than did control cells in vitro. Moreover, ERα knockdown in breast cancer cells resulted in decreased, even completely abrogated tumor growth in BALB/c nude mice, providing direct evidence for an essential role of ERα in breast cancer growth. Our results suggest siRNA-mediated gene silencing of ERα may impair tumorigenicity, and even suppress the tumor growth. ©2006 Landes Bioscience.