Correction: Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor (ACS Nano (2020) 14:4 (5135-5142) DOI: 10.1021/acsnano.0c02823)

Abstract

Here, we report three errors in the published article and provide corrected results. These corrections do not affect any conclusions of the work. CorrectiontoFigure2D,E.PanelsDandEofFigure2were reversed. We correct the error by switching the panels. Correction to Figure 6. We recently realized that the clinical samples we used in Figure 6B,C of the published article were not same condition as described in the text. We used clinicalsamplesstoredineNATinsteadofinuniversaltransport medium (UTM). Universal transport medium is for collection, transport, maintenance,and long-termfreezestorageofclinicalspecimens containing viruses and is suitable for cell culture, rapid antigen detection,PCR,andnucleicacidamplificationassays.eNATisa versatile molecular medium that is designed to stabilize and to preserve microbial nucleic acids and is optimized for molecular assays.eNAT contains guanidine−thiocyanate, which isused as a general protein denaturant, and this may interfere with antigen−antibody interactions. Afterwenoticedthissampleissue,weconductedexperiments toreplaceFigure6B,C.Wesuccessfullyconfirmedthatour fieldeffect transistor (FET) sensor clearly discriminated between patientandnormalsampleswithstatisticalsignificancein0.01× UTMcondition(see correctedFigure 6B,C),which isthe same condition for detecting antigen proteins in Figure 4E. We also performed repeated experiments for determining the limit of detectionofourFETsensor.Theresultswerequitethesameas our previous data, and we compensated for the defect of statistical significance (see corrected Figure 6D,E). In addition, we evaluated the previous results using eNAT clinicalsamples.WhenweappliedtheeNATsamplestotheFET sensor for a short time, the FET sensor could discriminate patientsamples(seeoriginalFigure6B,C).Whenweappliedthe eNATsampleforalongertime,however,theFETsensordidnot detect the patient samples, perhaps as a result of protein denaturation reagents in eNAT. Therefore, clinical samples in eNAT may not be suitable for viral antigen detection assay, although the antigen−antibody interactions may be maintained for a short time. Correction of typos. On page 5140, line 10, the text reads: However, the pristine graphene-based device without SARSCoV-2 spike protein conjugation did not show any remarkable signal change after the introduction of various sample concentration (gray line in Figure 4D). The control experiment indicates that the SARS-CoV-2 spike protein is essential for... The text should be changed to: However, the pristine graphene-based device without SARSCoV-2spikeantibodyconjugationdidnotshowanyremarkable signal change after the introduction of various sample concentration (gray line in Figure 4B). The control experiment indicates that the SARS-CoV-2 spike antibody is essential for...