Background/Aim. Fas/FasL system is a major regulator of apoptosis. The mechanisms by which Fas mediates cisplatin resistance remain unclear. The aim of this study is to explore the effect of Fas over-expression on cisplatin resistance of small cell lung cancer cells and its possible mechanisms. Materials and methods. Fas was over-expressed in H446/CDDP cells by infection with the adenoviruses containing Fas. Sensitivity of Fas-overexpressed H446/CDDP cells to cisplatin was evaluated using MTT assay. Expressions of Fas, GST-π and ERCC1 were detected by RT-PCR and Western blot analysis. Apoptosis rate was examined by FACS. Results: Over-expression of Fas in H446/CDDP cells significantly decreased the expressions of GST-π and ERCC1 at mRNA and protein levels, and increased the cell apoptosis. Furthermore, up-regulation of Fas significantly decreased the tolerance of H446/CDDP cells to cisplatin. Conclusion: Over-expression of Fas reverses drug resistance of H446/CDDP cells, possibly due to the increased cell sensitivity to apoptosis and the decreased expressions of GST-π and ERCC1. © 2010 Wu et al; licensee BioMed Central Ltd.
CITATION STYLE
Wu, W., Wang, H. D., Guo, W., Yang, K., Zhao, Y. P., Jiang, Y. G., & He, P. (2010). Up-regulation of fas reverses cisplatin resistance of human small cell lung cancer cells. Journal of Experimental and Clinical Cancer Research, 29(1). https://doi.org/10.1186/1756-9966-29-49
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