Biomolecule Detection

Abstract

The present invention relates to the detection of mols. of a target type in a sample. A method of detecting mols. of a target type in a sample comprises providing an aptamer-nucleic acid duplex, contacting the sample with the aptamer-nucleic acid duplex, wherein the aptamer is capable of selectively dissocg. from the nucleic acid to selectively bind to a mol. of the target type in the sample, amplifying any dissocd. nucleic acid and detecting any amplified nucleic acid. The method further comprises using the detected result to indicate the presence of mols. of the target type and/or quantify an amt. of mols. of the target type. Also provided is a system for detecting mols. of a target type in a sample. [on SciFinder(R)]