This chapter describes the development of paper-based microchip electrophoresis (pME) devices for the separation of clinically relevant compounds. pME were fabricated by laser cut and thermal lamination process using polyester pouches. In addition, hand-drawn pencil electrodes were integrated to the device to perform capacitively coupled contactless conductivity detection (C4D). Finished device costs less than US$ 0.10 and did not require either sophisticated instrumentation or clean room facilities. Furthermore, pME is lightweight, easy to handle, flexible, and robust. pME-C4D device revealed an excellent capacity to separate BSA and creatinine in less than 150 s with baseline resolution. The device proposed in this chapter has proven to be a good alternative as a platform for the diagnosis of diseases from renal disorders such as diabetes mellitus and heart disease.
CITATION STYLE
Chagas, C. L. S., Cardoso, T. M. G., & Coltro, W. K. T. (2019). Paper-based electrophoresis microchip as a powerful tool for bioanalytical applications. In Methods in Molecular Biology (Vol. 1906, pp. 133–142). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8964-5_10
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