[Irreversible chemical AFM-fishing for the detection of low-copied proteins].

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Abstract

The atomic-force microscopy-based method of irreversible chemical AFM-fishing (AFM-IF(Ch)) has been developed for the detection of proteins at ultra-low concentrations in solution. Using this method, a very low concentration of horseradish peroxidase (HRP) protein (10(-17) M) was detected in solution. A theoretical model that allows the description of obtained experimental data, is proposed. This model takes into consideration both the transport of the protein from the bulk solution onto the AFM-chip surface and its irreversible binding to the activated area.

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Ivanov, I. D., Danichev, V. V., Pleshakova, T. O., Shumov, I. D., Ziborov, V. S., Krokhin, N. V., … Archakov, A. I. (2014). [Irreversible chemical AFM-fishing for the detection of low-copied proteins]. Biomedit{combining Double Inverted Breve}sinskai{combining Double Inverted Breve}a Khimii{combining Double Inverted Breve}a, 60(1), 28–50. https://doi.org/10.18097/pbmc20146001028

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