Binding of the bovine caseinoglycopeptide to the platelet membrane glycoprotein GPIbα

Abstract

The bovine caseinoglycopeptide (residues 106-169), the C-terminal part of κ-casein, inhibited the von Willebrand factor-dependent platelet aggregation in a dose-dependent manner. An affinity matrix made of the caseinoglycopeptide selectively bound the platelet membrane glycoprotein GPIbα which contains the von Willebrand factor binding site. The amino acid residues of GPIbα participating in the caseinoglycopeptide binding were located after residue Glu 90.