An LC–MS/MS Method for Simultaneous Quantification of Seven Anti-HIV Medicines in Plasma of HIV-infected Patients

Abstract

A quick and high-through LC-MS/MS method has been developed and applied to simultaneously quantify lamivudine (3TC), stavudine (d4T), zidovudine (AZT), efavirenz (EFV), nevirapine (NVP), and lopinavir/ritonavir (LPV/RTV) plasma concns. A combination of protein pptn. and liq.-liq. extn. was used to ext. all compds. The method showed a good linearity in a concn. range of 20-3200 μg/L for 3TC, d4T, AZT, 40-6400 μg/L for EFV and NVP, 62.5-10000 μg/L for LPV, and 12.5-2000 μg/L for RTV. Mean intra- and inter-day precision were within ±20% at the LLOQ and ±15% at the other QC level. The accuracy were between 85% and 115% for all seven analytes. The whole run is 13 min. This method has been successfully used for analyzing 133 samples from 84 HIV-pos. patients being treated with the combination therapy in China. It is the 1st time to use LC-MS/MS method to simultaneously quantify 3TC, d4T, AZT, EFV, NVP, LPV, and RTV in China for investigating the relationship between blood drug concns. and efficacy and/or toxicity. [on SciFinder(R)]