RNA aptamers for targeting mitochondria using a mitochondria-based SELEX method

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Abstract

The use of mitochondria-based systematic evolution of ligands by exponential enrichment (SELEX) was explored. Mitochondria were isolated from rat liver and confirmed intact by respiratory control index. Isolated mitochondria and a 2′-F RNA random library were mixed and the bound RNAs collected. The counter selection was applied with nucleus and unbound RNAs were collected. After 7 rounds of selection, two sequences (Mitomer1 and Mitomer2) were verified to bind to mitochondria and the truncated Mitomer2 (short Mitomer2) showed better binding to isolated mitochondria than Mitomer1.

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Tawaraya, Y., Hyodo, M., Ara, M. N., Yamada, Y., & Harashima, H. (2014). RNA aptamers for targeting mitochondria using a mitochondria-based SELEX method. Biological and Pharmaceutical Bulletin, 37(8), 1411–1415. https://doi.org/10.1248/bpb.b14-00112

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