Physical constraints determine the logic of bacterial promoter architectures

19Citations
Citations of this article
74Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Site-specific transcription factors (TFs) bind to their target sites on the DNA, where they regulate the rate at which genes are transcribed. Bacterial TFs undergo facilitated diffusion (a combination of 3D diffusion around and 1D random walk on the DNA) when searching for their target sites. Using computer simulations of this search process, we show that the organization of the binding sites, in conjunction with TF copy number and binding site affinity, plays an important role in determining not only the steady state of promoter occupancy, but also the order at which TFs bind these effects can be captured by facilitated diffusion-based models, but not by standard thermodynamics. We show that the spacing of binding sites encodes complex logic, which can be derived from combinations of three basic building blocks: switches, barriers and clusters, whose response alone and in higher orders of organization we characterize in detail. Effective promoter organizations are commonly found in the E coli genome and are highly conserved between strains. This will allow studies of gene regulation at a previously unprecedented level of detail, where our framework can create testable hypothesis of promoter logic. © 2014 The Author(s). Published by Oxford University Press.

Cite

CITATION STYLE

APA

Ezer, D., Zabet, N. R., & Adryan, B. (2014). Physical constraints determine the logic of bacterial promoter architectures. Nucleic Acids Research, 42(7), 4196–4207. https://doi.org/10.1093/nar/gku078

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free