Production of anti-amyloid /? Antibodies in mice fed rice expressing amyloid /?

Abstract

The main signs of Alzheimer's disease (AD) are cognitive impairment and senile plaques composed of amyloid beta (A/3) observed in patients' brains. Therefore, therapy for AD focuses on the removal of A/?. We developed an "edible vaccine" that employs intestinal immunity with little to no side effects. Rice was utilized as an edible vaccine. It expressed GFP-A/342. A/} rice was administered orally to wild-type (WT) mice causing production of anti-A/3 antibodies. Since A/} rice was mixed with the cholera toxin B subunit (CTB), antibody against the rice seed protein was also produced. Then, mice were caused to develop immune tolerance against the rice seed protein by oral administration of A/? rice mixed with CTB. The results indicated that only anti-A/? antibodies were produced.