The magnitude and breadth of T-cell responses against HCV are associated with the outcome of HCV infection. Parameters of HCV-specific T-cell responses that are frequently assessed in clinical immunological studies include proliferation of T cells in response to HCV antigens, frequency of HCV-specific T cells secreting cytokines, and changes in antigen specificity during the course of HCV infection. Common techniques for assessing these parameters such as (3)H-thymidine incorporation assay, cytokine ELISpot assay, and strategies for epitope mapping and identification of minimal optimal epitopes are outlined, and detailed protocols are described.
CITATION STYLE
Yoon, J. C., & Rehermann, B. (2009). Determination of HCV-specific T-cell activity. Methods in Molecular Biology (Clifton, N.J.), 510, 403–413. https://doi.org/10.1007/978-1-59745-394-3_30
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