Phytochemical identification from boehmeria nivea leaves and analysis of (–)-loliolide by HPLC

Abstract

Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as β-sitosterol, (−)-loliolide, rutin, and pyrimidinedione by MS, 1H-, and 13C-NMR spectroscopic analysis. Among them, (−)-loliolide was isolated for the first time from B. nivea. A content analysis of (−)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (−)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.