Genetic polymorphisms in genes encoding antioxidant enzymes are associated with diabetic retinopathy in type 1 diabetes

53Citations
Citations of this article
41Readers
Mendeley users who have this article in their library.

Abstract

OBJECTIVE - Oxidative stress plays an important role in the development of microangiopathic complications in type 1 diabetes. We investigated polymorphic markers in genes encoding enzymes regulating production of reactive oxygen species in association with diabetic retinopathy or diabetic nephropathy. RESEARCH DESIGN AND METHODS - A total of 124 patients with type 1 diabetes were investigated in this case-control study. All subjects were matched for sex, age, and duration of diabetes. Genotyping was conducted using real-time PCR for p.Val16Ala polymorphism in the MnSOD gene and c.C-262T in the promoter region of the CAT gene. Multiplex PCR method was used for determination of GSTM1 and GSTT1 polymorphic deletions. Fluorescence-labeled PCR amplicons and fragment analysis was used for assessing the number of pentanucleotide (CCTTT)n repeats in inducible nitric oxide synthase. RESULTS - A positive association of MnSOD genotype Val/Val (odds ratio [OR] 2.49, 95% CI 1.00-6.16, P = 0.045) and GSTM1-1 genotype (2.63, 1.07- 6.47, P = 0.031) with diabetic retinopathy but not with diabetic nephropathy was demonstrated. Additionally, the combination of the two genotypes conveyed an even higher risk (4.24, 1.37-13.40, P = 0.009). No other investigated genetic polymorphisms were associated with either diabetic retinopathy or diabetic nephropathy. CONCLUSIONS - Selected polymorphisms in genes encoding MnSOD and GSTM1 could be added to a panel of genetic markers for identification of individuals with type 1 diabetes at an increased risk for developing diabetic retinopathy. © 2009 by the American Diabetes Association.

References Powered by Scopus

The pathobiology of diabetic complications: A unifying mechanism

4472Citations
N/AReaders
Get full text

Report of the expert committee on the diagnosis and classification of diabetes mellitus

3416Citations
N/AReaders
Get full text

Oxidative stress and diabetic vascular complications

1744Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Manganese superoxide dismutase and oxidative stress modulation

229Citations
N/AReaders
Get full text

Evaluation of oxidative stress markers in pathogenesis of diabetic neuropathy

118Citations
N/AReaders
Get full text

Diabetic retinopathy, superoxide damage and antioxidants

90Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Hovnik, T., Dolžan, V., Bratina, N. U., Podkrajšek, K. T., & Battelino, T. (2009). Genetic polymorphisms in genes encoding antioxidant enzymes are associated with diabetic retinopathy in type 1 diabetes. Diabetes Care, 32(12), 2258–2262. https://doi.org/10.2337/dc09-0852

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 12

50%

Professor / Associate Prof. 7

29%

Researcher 4

17%

Lecturer / Post doc 1

4%

Readers' Discipline

Tooltip

Medicine and Dentistry 11

44%

Agricultural and Biological Sciences 7

28%

Biochemistry, Genetics and Molecular Bi... 4

16%

Neuroscience 3

12%

Save time finding and organizing research with Mendeley

Sign up for free