The planar lipid bilayer method is another way to examine channel molecules functionally at the single-molecule level. In contrast to patch-clamping, channel molecules are isolated from various biological resources and are reconstituted into an artificial membrane that has a defined lipid composition. Various techniques have been developed, from the conventional painting method to liposome-patch clamping. In this chapter, underlying principles and technical details for forming the planar lipid bilayer and methods for incorporating channel molecules into the bilayer are reviewed.
CITATION STYLE
Oiki, S. (2012). Planar Lipid Bilayer Method for Studying Channel Molecules (pp. 229–275). https://doi.org/10.1007/978-4-431-53993-3_16
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